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Toll Free: 1-888-71-(86583)
Local Phone: 360-734-1789
Fax: 360-734-0798
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Europe
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Local Phone: +31 (0)10 2232 774
Fax: +31 (0)10 2236 212
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3068JP Rotterdam

Hygromycin B EvoPure®

Product overview

HPLC Analysis of Hygromycin B from different suppliers

HPLC Major Impurities (Over 1%) for different supplier's Hygromycin B Identified as different rention times

HPLC Retention Time Batch of Hygromycin B Impurities of Hygromycin B
  Supplier A (%) Supplier B (%) Supplier C (%) TOKU-E(%) EvoPure®(%)  
6.0 min 2.17 -- 2.27 -- -- A
6.9 min 5.11 1.0 5.97 -- -- C
7.1 min 1.59 -- -- -- -- D
7.4 min 6.54 1.75 5.92 1.2 0.5 E
8.1 min 18.5 14.35 15.4 1.55 -- F
8.6 min 64.9 79.4 66.7 94.7 99.0 Hygromycin B

Survival rate of resistant cells at high concentrations hygromycin B (300 μg/mL)

  Supplier A Supplier B TOKU-E normal Hygromycin EvoPure®
Tobacco Calli cells transformed
with the Hygromycin B
phosphotransferase gene
2 6 86 100
293 cells transformed
with the Hygromycin B phosphotransferase gene
1 8 91 100